Panobinostat Treatment in Symptomatic Mice

Symptomatic mice (BSG GEMM) were treated with three doses of 20 mg/kg panobinostat (Selleckchem) or vehicle (25% DMSO, 0.25x PBS, 5% glucose) (n = 3 in each group) administered once daily by intraperitoneal injections. Mice were sacrificed 4 h after their final treatment via CO₂. Brains from the sacrificed mice were extracted. Half of each brain was fixed in 10% formalin and embedded in paraffin for histological analysis. The other half was SNAP frozen, stored at -80 degrees and sent for pharmacokinetic (PK) studies. Pharmacokinetic analysis was performed on the cerebral cortex tissue and brainstem tumor tissue of each mouse by the Pharmacokinetic/Pharmacodynamic (PK/PD) Core Laboratory, Duke Cancer Institute as described below. Statistical significance was determined using unpaired two-tailed t-test to compare groups.