Temporal Dynamics of VEGF and SDF-1α in HLI

Concentrations of vascular endothelial growth factor (VEGF) and stromal cell–derived factor‐1 (SDF‐1) α proteins in murine blood plasma samples were determined by ELISA kits (mouse VEGF ELISA kit, R&D Systems, and mouse CXCL12/SDF‐1α Quantikine ELISA kit, respectively), according to manufacturer's instructions.¹⁸ (link) Plasma levels of VEGF and SDF‐1α in sham operation and at day 7 after HLI surgery were measured every 6 hours (6:00, 12:00, 18:00, and 24:00) in both the control and jet‐lag groups.

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Tsuzuki K., Shimizu Y., Suzuki J., Pu Z., Yamaguchi S., Fujikawa Y., Kato K., Ohashi K., Takefuji M., Bando Y.K., Ouchi N., Calvert J.W., Shibata R, & Murohara T. (2021). Adverse Effect of Circadian Rhythm Disorder on Reparative Angiogenesis in Hind Limb Ischemia. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(16), e020896.

Publication 2021

mouse VEGF ELISA kit mouse CXCL12/SDF‐1α Quantikine ELISA kit

Blood plasma proteins Cxcl12 Elisa Jet lag Mouse Plasma Stromal cell derived factor 1 α Surgery Vascular endothelial growth factor

Corresponding Organization :

Other organizations : Nagoya University, Suzuki (Japan), Emory University Hospital Midtown

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Variable analysis

independent variables

Jet-lag group

dependent variables

Plasma levels of VEGF
Plasma levels of SDF-1α

control variables

Sham operation group

controls

Positive control: Sham operation group
Negative control: Not explicitly mentioned

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