Tellurite Reductase Activity Assay

TR activity was determined in 200 μl reactions using 10 μl of cell free extract in buffer 50 mM Tris-HCl (pH 7.4), 1 mM tellurite, 1 mM NAD(P)H (Sigma-Aldrich) and 1 mM β-mercaptoethanol (Sigma-Aldrich). TR activity was monitored following the appearance of tellurium at 500 nm as described previously57 (link). One unit (U) of TR activity was defined as the amount of enzyme required to increase the OD 500 nm in 0.001 min⁻¹ mg protein⁻¹.

Free full text: Click here

Morales E.H., Pinto C.A., Luraschi R., Muñoz-Villagrán C.M., Cornejo F.A., Simpkins S.W., Nelson J., Arenas F.A., Piotrowski J.S., Myers C.L., Mori H, & Vásquez C.C. (2017). Accumulation of heme biosynthetic intermediates contributes to the antibacterial action of the metalloid tellurite. Nature Communications, 8, 15320.

Publication 2017

tellurite NAD(P)H β-mercaptoethanol

Buffer Cell extract Enzyme Mercaptoethanol Nad h Protein 1 Tellurite Tellurium Tris

Corresponding Organization : Nara Institute of Science and Technology

Other organizations : Universidad de Santiago de Chile, University of Minnesota, Yumanity Therapeutics (United States)

Top 5 similar protocols

Variable analysis

independent variables

Tellurite concentration
NAD(P)H concentration
β-mercaptoethanol concentration

dependent variables

TR activity (as measured by increase in OD at 500 nm)

control variables

Buffer (50 mM Tris-HCl, pH 7.4)
Volume of cell-free extract (10 μl)
Reaction volume (200 μl)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!