Protocol detail

CTCL Cell Line Culture Protocol

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The CTCL cell lines SeAx [50 (link)], Hut78 [51 (link)], MyLa [52 (link)], and HH [53 (link)] were cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FCS and 1 mM L-Glutamine. SeAx, MyLa, and HH cells were obtained from Stefan Eichmüller, German Cancer Research Center, Heidelberg, Germany. Hut78 cells were purchased from ATCC. SeAx, Hut78, MyLa, and HH cells were tested by the cell contamination control of German Cancer Research Center, Heidelberg, Germany using a multiplex cell contamination test [54 (link)].

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KieΔling M.K., Nicolay J.P., Schlör T., Klemke C.D., Süss D., Krammer P.H, & Gülow K. (2017). NRAS mutations in cutaneous T cell lymphoma (CTCL) sensitize tumors towards treatment with the multikinase inhibitor Sorafenib. Oncotarget, 8(28), 45687-45697.

Publication 2017

Hut78

Cancer Cell Cell lines Ctcl Cultured medium Glutamine

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Variable analysis

independent variables

Cell lines: SeAx, Hut78, MyLa, and HH

dependent variables

Not explicitly mentioned

control variables

Culture medium: Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FCS and 1 mM L-Glutamine
Cell contamination control: Multiplex cell contamination test performed by the German Cancer Research Center, Heidelberg, Germany

controls

Positive control: Not mentioned
Negative control: Not mentioned

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