Cefuroxime axetil-loaded PLA-HPG nanoparticles

Cefuroxime axetil was provided by Meilun (Dalian, Liaoning, China). Polylactic acid (PLA) (Mw = 17 kDa) was obtained from SunLip Biotechnology (Shanghai, China). PLA-HPG was synthesized as previously described (Deng et al., 2014 (link); Deng et al., 2015 (link)). Cyanine 5 (Cy5) and cyanine 7.5 (Cy7.5) were ordered from Aladdin (Shanghai, China). NaIO₄, Na₂SO₃, anhydrous dichloromethane (DCM), sodium dodecyl sulfate (SDS), and HPLC-grade methanol were obtained from Macklin (Shanghai, China). N, N′-diisopropylcarbodiimide (DIC) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Potassium dihydrogen phosphate and potassium ammonium dihydrogen phosphate were chemical reagents (Guangzhou, Guangdong, China).