Antibody Profiling for Cell Cycle Regulation

Commercially available antibodies obtained from Cell Signaling against the indicated proteins were: Cdc2/CDK1 (Cat # 9112S), CDK1-P-Y15 (Cat # 4539S), Histone H3-P-S28 (Cat # 9713S), p53-P-S15 (Cat # 9284), SMC1-P-957 (Cat # 4805S), SMC1 (Cat # 4802S), CDC25A (Cat # 3652S) and Chk2-P-T68 (Cat # 2661S). Antibodies obtained from Santa Cruz Biotechnology were CDC25A F-6 (Cat # sc -7389), CDC25C H-150 (Cat # sc-5620), Cdc2 (Cat # sc-54). Others were against actin (Cat. 109 # MA515739, Pierce), RPA32-P-S4/8 (Cat # A300-245A, Bethyl), cyclin A (Cat # 06-138, Upstate) γH2AX (Cat # 05-636, Millipore), Chk2 Clone 7 (Cat # 05-649, Millipore), cyclin B1 (Cat. # 05-373, Millipore), and CDK1 (Cat. #Ab18, Abcam). Secondary antibodies have previously been described. Additional antibodies used for immunofluorescence include: cyclin B1 (Cat # 4138S, Cell signaling), Alexa 488 (anti-mouse Cat # A11029, anti-rabbit Cat # A11034, Invitrogen) and Alexa 568 (anti-mouse Cat # A11031, anti-rabbit Cat # A11036, Invitrogen). NS1 CE10 and NS1 91W were described previously [7] (link).

Free full text: Click here

Adeyemi R.O, & Pintel D.J. (2014). Parvovirus-Induced Depletion of Cyclin B1 Prevents Mitotic Entry of Infected Cells. PLoS Pathogens, 10(1), e1003891.

Publication 2014

Cdc2/CDK1 CDK1-P-Y15 p53-P-S15 CDC25A Chk2-P-T68 CDC25A F-6 RPA32-P-S4/8 γH2AX cyclin B1 Alexa 488 Alexa 568

Actin Alexa 568 Antibodies Cdc25a Cdc25c Cdk1 Clone Cyclin a Cyclin b1 Histone h3 Immunofluorescence Mouse Proteins Rabbit

Corresponding Organization :

Other organizations : University of Missouri

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of Cdc2/CDK1
Levels of CDK1-P-Y15
Levels of Histone H3-P-S28
Levels of p53-P-S15
Levels of SMC1-P-957
Levels of SMC1
Levels of CDC25A
Levels of Chk2-P-T68
Levels of actin
Levels of RPA32-P-S4/8
Levels of cyclin A
Levels of γH2AX
Levels of Chk2
Levels of cyclin B1
Levels of CDK1

control variables

None explicitly mentioned

positive controls

NS1 CE10
NS1 91W

negative controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!