Flow Cytometry Panel for Immune Cell Profiling

Flow cytometry antibodies: B220/CD45R-e450, CD3-e780, CD11b-APC, CD11c-e780, CD19-e780, CD49b-e780 CD86-Alexa Fluor 488, NK1.1-e780 from eBioscience. CD8α-PECF594, CD40-PE from BD Pharmingen. B220-BV650, CD11b-BV711, CD11c-BV421, CD40-FITC, CD80-BV605, CD86-A700, F4/80-PE-Cy7, Ly6C-BV570, Ly6G-APC-Cy7, MHC II-PerCP-Cy5.5 from Biolegend. PDCA-1-PE, FcR block from Miltenyi Biotec. The S9.6 monoclonal antibody against RNA:DNA hybrids was purified from hybridoma cell line HB-8730 (ATCC-LGC Promochem) supernatant using a Protein A/G column as previously described (Pohjoismaki et al, 2010 (link)).

Free full text: Click here

Rigby R.E., Webb L.M., Mackenzie K.J., Li Y., Leitch A., Reijns M.A., Lundie R.J., Revuelta A., Davidson D.J., Diebold S., Modis Y., MacDonald A.S, & Jackson A.P. (2014). RNA:DNA hybrids are a novel molecular pattern sensed by TLR9. The EMBO Journal, 33(6), 542-558.

Publication 2014

CD3-e780 CD11b-APC CD8α-PECF594 CD40-PE B220-BV650 CD11b-BV711 CD11c-BV421 CD40-FITC F4/80-PE-Cy7 Ly6G-APC-Cy7 MHC II-PerCP-Cy5.5 FcR block

Alexa fluor 488 Antibodies Cd11b Cell line Cy5 5 Fitc Flow cytometry Hybridoma Hybrids Monoclonal antibody Pdca 1 Protein g

Corresponding Organization :

Other organizations : University of Edinburgh, Yale University, King's College London

Top 5 similar protocols

Variable analysis

independent variables

B220/CD45R-e450
CD3-e780
CD11b-APC
CD11c-e780
CD19-e780
CD49b-e780
CD86-Alexa Fluor 488
NK1.1-e780
CD8α-PECF594
CD40-PE
B220-BV650
CD11b-BV711
CD11c-BV421
CD40-FITC
CD80-BV605
CD86-A700
F4/80-PE-Cy7
Ly6C-BV570
Ly6G-APC-Cy7
MHC II-PerCP-Cy5.5
PDCA-1-PE

dependent variables

Not explicitly mentioned

control variables

FcR block from Miltenyi Biotec
S9.6 monoclonal antibody against RNA:DNA hybrids purified from hybridoma cell line HB-8730 (ATCC-LGC Promochem) supernatant using a Protein A/G column as previously described (Pohjoismaki et al, 2010)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!