Individual carotenoid content was determined using RP-HPLC, which was performed using a Shimadzu LC-20AT with an SPD-M20A diode array detector (DAD) (Shimadzu Corporation, Kyoto, Japan), as described by Ghendov-Mosanu et al. [10 (link)]. Comparison of the UV–Vis spectra and the retention times of the sample peaks with those of the standard solutions and spectral data reported in the literature allowed for the identification of carotenoids from sea buckthorn samples (Table 5).
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