L-363, U-266, Daudi, Raji and CHO-K1 cells were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; cat. no. ACC 49, ACC 9, ACC 78, ACC 110), whereas CEM were purchased from the American Type Culture Collection (ATCC; cat. no. CCL-119). L-363, U-266, Raji, Daudi and CEM cells were cultured in RPMI 1640 GlutaMAX medium containing 10% FCS, 1% penicillin and streptomycin (R10+; all Thermo Fisher Scientific; cat. no. 72400-021, 10270-106, 15140-122). CHO-K1 cells were cultured in DMEM medium containing 10% FCS, 1% penicillin and streptomycin (D10+; all Thermo Fisher Scientific; cat. no. 41965-039, 10270-106, 15140-122). HUVEC and CHO-S cells were ordered from Lonza (cat. no. CC-2519) and Thermo Fisher Scientific (cat. no. R800-07), respectively, and cultured according to manufacturer’s instructions. The human plasma cell line INA-6 was cultured in RPMI 1640 GlutaMAX medium containing 20% FCS, 1% penicillin and streptomycin supplemented with 10 ng/ml recombinant human IL-6 (Thermo Fisher Scientific; cat. no. PHC0061) (25 (link)). Stable transfected CHO-K1 cells expressing myeloma antigens were generated and cultured as described (23 (link)).
Free full text: Click here
