Lead Exposure in Tg-SwDI Mice

Tg-SwDI (APP-Swedish, Dutch, Iowa) mice were introduced from the Jackson Laboratory (Bar Harbor, ME, USA) and bred in the laboratory of Animal Center at the Indiana University School of Medicine (Indianapolis, IN, USA). All procedures and materials used in this animal study were approved by the Institutional Animal Care and Use Committee (IACUC) at the Indiana University School of Medicine. Five-month-old female mice were housed, 3 to 5 each cage, supplied ad libitum with food and water, in a 12 h light/dark cycle facility. In this study, three mice orally received 27 mg Pb/kg (i.e., 50 mg Pb-acetate/kg) (Sigma-Aldrich Corporation, St. Louis, MO, USA) daily by gavage (Pb-treated group). The control group orally received an equivalent molar concentration of Na-acetate daily [39 (link)]. All mice were sacrificed at 30 days after the first oral treatment.