RNase And proteinase combined with Triton X-100 were used to eliminate EVs-free and intra-EVs RNAs and proteins. EVs were primarily treated with 100 μg/mL proteinase K (TIANGEN, cat. RT403, Beijing, China) at 37°C for 30min, followed by 10μg/mL RNase A (TIANGEN, cat. RT405, Beijing, China) at 37°C for 15min, additionally 0.1% Triton X-100 was added between RNase A and proteinase K treatment 20 (link).
Free full text: Click here