Three sealers have been selected for the current study; Ceramoseal Bioceramic sealer (DM Trust, Minya, Egypt) which is a bioceramic-based single-paste hydrophilic root canal sealer. It is composed of silicon dioxide, aluminum oxide calcium oxides, titanium dioxide, barium sulfate, calcium sulfate, and calcium carbonate in special particle size distribution of Nano and micro size. The other two sealers were Totalfill BC sealer (FKG, La Chaux-de-Fond, Switzerland) and AH plus epoxy resin sealer (Dentsply, New York, USA).