Glycated haemoglobin (HbA1c) was determined by liquid chromatography on an Adams A1C HA-8160 analyser (Arkray, Kyoto, Japan); HbA1c values were converted to units aligned with the Diabetes Control and Complications Trial, according to the US National Glycohemoglobin Standardization Program [47 (link)].
Fructosamine levels were determined by the diazyme glycated serum protein enzymatic method (Diazyme, Kent, UK) on an Advia 2400 analyser (Siemens). This assay uses protein K to digest serum proteins into small fragments and fructosaminase to catalyse the specific oxidation of the ketoamine linkage of glycated fragments. The release of hydrogen peroxide, measured colorimetrically at 550 nm, is proportional to the concentration of glycated serum proteins (fructosamine). To calculate the percentage of glycated albumin, the values of fructosamine (glycated serum proteins) were determined using the aforementioned method and those of albumin using the bromocresol green method. The glycated albumin percentage was calculated following the equation recommended by the manufacturer [48 (link)].
Fructosamine levels were determined by the diazyme glycated serum protein enzymatic method (Diazyme, Kent, UK) on an Advia 2400 analyser (Siemens). This assay uses protein K to digest serum proteins into small fragments and fructosaminase to catalyse the specific oxidation of the ketoamine linkage of glycated fragments. The release of hydrogen peroxide, measured colorimetrically at 550 nm, is proportional to the concentration of glycated serum proteins (fructosamine). To calculate the percentage of glycated albumin, the values of fructosamine (glycated serum proteins) were determined using the aforementioned method and those of albumin using the bromocresol green method. The glycated albumin percentage was calculated following the equation recommended by the manufacturer [48 (link)].
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