Evaluating LDB1 Knockdown on P388D1 Tumor Growth

Based on past experience [24 (link)] 4–6 week-old BALB/c female mice were randomly divided into two groups: sh-NC and sh-Ldb1, and each group contains seven mice. The P388D1 cells expressing firefly luciferase (P388D1.ffluc) were transfected with sh-NC or sh-LDB1 and were injected into the tail vein with 2 × 10⁵ cells suspensions in 100ul PBS for each mice . D6/9/12 days after tail vein injection, D-luciferin sodium salt (115144-35-9, GOLDBIO, USA) was injected into the abdominal cavity of tumor-bearing mice, and small animal live imager (Berthold, Germany) scans were performed to measure the maximum standard Avg radiance uptake value of tumors in mice. Animals were anesthetized with 2% isoflurane and set for the acquisition of PET scan within 30 min. Mice were first euthanized by inhalation of CO₂, and tissues, such as liver, spleen and bones were collected for subsequent experimental procedures, including paraffin block and HE staining. IHC was performed as previously described [25 (link)]. Primary antibodies against Ki67(GB12114, Servicebio, China) were used according to the manufacturer’s recommendations.