m6A-Containing mRNA Enrichment and Sequencing

50 μg of total RNA was extracted and purified using PolyTtract mRNA Isolation System (Promega, Hong Kong). After fragmentation, RNA was incubated with an anti-m6A antibody for one hour at 4°C, and then mixed with prewashed Protein A/G Magnetic Beads (Merck Millipore, Germany) in immunoprecipitation buffer at 4°C overnight. Enrichment of m6A containing mRNA was purified for further MeRIP sequencing by RiboBio (Guangzhou, China). RNA-seq was conducted in accordance with a previously reported protocol (13 (link)). Fold change of >1.5 and false discovery rate P < 0.05 were set as the cutoffs to screen for differentially expressed genes (DEGs).