Single-cell RNA-seq library preparation

Single-cell suspensions (1~3×105 cells/mL) in PBS (HyClone) were loaded onto microwell chip using the Singleron Matrix® Single Cell Processing System. Briefly, the scRNA-seq library was constructed using the GEXSCOPE® Single Cell RNA Library Kits (Singleron). The library was lastly sequenced with 150 bp that was diluted to 4nM and paired-end reads on the IlluminaHiSeq X platform following an established protocol (7 (link)). Sequencing data processing and quality control was performed as described in previous publications (8 (link), 9 (link)).

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Yu B., Jing P., Gao F., Zhang P., Zheng G, & Zhang X. (2024). Effect of sodium stibogluconate in recruiting and awakening immune cells in the pleural fluid of pancreatic cancer: preparation for immunotherapy. Frontiers in Immunology, 14, 1315468.

Publication 2023

Matrix® Single Cell Processing System GEXSCOPE® Single Cell RNA Library Kits

Corresponding Organization : Peking University Cancer Hospital

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Variable analysis

independent variables

Single-cell suspensions (1~3×10^5 cells/mL) in PBS (HyClone)

dependent variables

ScRNA-seq library construction using the GEXSCOPE® Single Cell RNA Library Kits (Singleron)
Sequencing of the library with 150 bp that was diluted to 4nM and paired-end reads on the IlluminaHiSeq X platform

control variables

Microwell chip using the Singleron Matrix® Single Cell Processing System
Sequencing data processing and quality control as described in previous publications (8, 9)

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