4’, 6-diamidino-2-phenylindole (DAPI) was purchased from Fujifilm Wako. Antibodies were obtained as follows: FLAG M2 mouse monoclonal antibody (1:3000 dilution; F1804, Sigma-Aldrich Japan, Tokyo, Japan), rabbit anti-β-actin antibody (1:3000 dilution; A2066, Sigma-Aldrich Japan), rabbit anti-HNRNPM antibody (1:2000 dilution; HPA024344, Sigma-Aldrich Japan) and mouse anti-SRRM2 antibody (1:2000 dilution; S4045, Sigma-Aldrich Japan), HA (12CA5) mouse monoclonal antibody (1:2000 dilution; GTX16918, GeneTex, Irvine, CA), mouse anti-GAPDH antibody (1:2000 dilution; 016-25523, Fujifilm Wako), sera against THOC1 (1:1000 dilution), THOC2 (1:1000 dilution), THOC5 (1:1000 dilution), ALYREF (1:1000 dilution), CIP29 (1:1000 dilution), UAP56 (1:1000 dilution) and URH49 (1:1000 dilution) have been described previously11 (link). Anti-RUVBL1 (1:1000 dilution), anti-RUVBL2 (1:1000 dilution), anti-ILF2 (1:1000 dilution), and anti-ILF3 sera (1:1000 dilution) were prepared from immunized Wistar female rats as described previously59 (link) in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Animal Committee in Kyoto University (Animal experiments were approved by the Committee on the Ethics of Animal Experiments of Kyoto University, Experiment permission number: Lif-K17002). The antibodies used in this study are listed in Supplementary Table 4.
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