Estradiol (E2, 1,3,5(10)-estratrien-3,17β diol) and sulforhodamine B (SRB) were purchased from Sigma Aldrich (St. Louis, MO, USA). The 17β-AEs, prolame [17β-(3-hydroxy-1-propylamine)-1,3,5(10)-estratrien-3-ol)], butolame [17β-(3-hydroxy-1-butylamine)-1,3,5(10)-estratrien-3-ol)], and pentolame [17β-(5-hydroxy-1-pentolamine)-1,3,5(10)-estratrien-3-ol)], were prepared from estrone according to previously reported methods [21 (link),44 (link)]. G36 (4-(6-bromo-benzo[1,3]dioxol-5-yl)-8-isopropyl-3a,4,5,9b-tetrahydro-3H-cyclopenta quinoline) was synthesized and characterized according to the procedure reported by Denis et al. [43 (link)].
The compounds were synthesized following exactly the reported procedures [21 (link),44 (link)]. E2, the 17β-aminoestrogens, and G36 were dissolved in absolute ethanol and used as a stock solution (Merck, Darmstadt, Germany). All cell culture reagents and media, as well as the molecular biology reagents, were purchased from Gibco (Invitrogen Corporation, Waltham, MA, USA).
Additionally, 7-Aminoactinomycin D (7-AAD) was acquired from BioLegend, San Diego, CA, USA, Phosflow Fix Buffer I and Phosflow Perm Buffer III were purchased from Becton Dickinson (BD) Biosciences, San Jose, CA, USA), and the rabbit anti-phospho c-fos (SER32) monoclonal antibody was acquired from Cell Signaling Technology, Danvers, MA, USA.
The compounds were synthesized following exactly the reported procedures [21 (link),44 (link)]. E2, the 17β-aminoestrogens, and G36 were dissolved in absolute ethanol and used as a stock solution (Merck, Darmstadt, Germany). All cell culture reagents and media, as well as the molecular biology reagents, were purchased from Gibco (Invitrogen Corporation, Waltham, MA, USA).
Additionally, 7-Aminoactinomycin D (7-AAD) was acquired from BioLegend, San Diego, CA, USA, Phosflow Fix Buffer I and Phosflow Perm Buffer III were purchased from Becton Dickinson (BD) Biosciences, San Jose, CA, USA), and the rabbit anti-phospho c-fos (SER32) monoclonal antibody was acquired from Cell Signaling Technology, Danvers, MA, USA.
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