Visualizing Meiotic Chromosome Structures

Pachytene chromosome spreads were prepared from testes according to Moens (2006) (link) and from ovaries following Araya-Jaime et al. (2015) (link). Synaptonemal complexes (SCs) were visualized using immunofluorescent staining with antibodies against SYCP3 (ab14206; Abcam) and SYCP1 (a gift from Sean M. Burgess). The recombination sites were visualized by antibody staining against the MLH1 (ab15093; Abcam) proteins. Detailed descriptions of pachytene chromosome preparation and immunofluorescent staining are given in File S1.

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Dedukh D., Majtánová Z., Marta A., Pšenička M., Kotusz J., Klíma J., Juchno D., Boron A, & Janko K. (2020). Parthenogenesis as a Solution to Hybrid Sterility: The Mechanistic Basis of Meiotic Distortions in Clonal and Sterile Hybrids. Genetics, 215(4), 975-987.

Publication 2020

ab14206 ab15093

Antibodies Antibody Chromosome Immunofluorescent Mlh1 Ovaries Proteins Recombination Synaptonemal complexes Testes

Corresponding Organization : University of Ostrava

Other organizations : University of Warmia and Mazury in Olsztyn, University of South Bohemia in České Budějovice, University of Wrocław

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Tissue type (testes, ovaries)

dependent variables

Synaptonemal complex (SC) visualization
Recombination site visualization

control variables

Antibodies used for immunofluorescent staining (SYCP3, SYCP1, MLH1)

controls

Positive control: None specified
Negative control: None specified

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