Profiling Anticancer Compounds via Spectroscopic Analysis

HRESIMS spectra were acquired on Micromass Q-ToF spectrometer (Waters Corporation, Milford, MA, USA). NMR spectra were obtained on Bruker Avance DRX 400 and 600 MHz (Bruker, Rheinstetten, Germany). The UV spectra were measured on a Hitachi 300 spectrometer (Hitachi High-Technologies Corporation, Kyoto, Japan) and as reported earlier [34 (link)]. Liquid–Liquid partitions, C18 Sep-Pak and Sephadex LH-20 were used for fractionation of the extract and successive fractions. A Gemini^® C18 column (5 µm, Phenomenex, 250 × 0.64 mm) (Torrance, CA, USA) was used for HPLC purification of the compounds. The HCT 116, MDA-MB-231 cell lines were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA), while NHDF cell line was obtained from PromoCell GmbH (Heidelberg, Germany).