Cholangiocytes were treated with H1HR or H2HR antagonists and proliferation was measured by MTS assay and BrdU incorporation (15 (link), 16 (link), 22 (link)).
Similar to our in vivo studies, we performed experiments to determine the effects of blocking either H1HR or H2HR on CCA proliferation by MTS assay, and angiogenesis and EMT by real-time PCR. CCA cells were treated for up to 24 hours with vehicle, mepyramine or ranitidine prior to MTS assay and real-time PCR. Invasion was measured using a commercially available kit following vehicle, H1HR or H2HR antagonist treatment. The QCM ECMatrix Cell Invasion Assay was purchased from EMD Millipore (Billerica, MA) and used per manufacturer’s instructions (13 (link)).
Similar to our in vivo studies, we performed experiments to determine the effects of blocking either H1HR or H2HR on CCA proliferation by MTS assay, and angiogenesis and EMT by real-time PCR. CCA cells were treated for up to 24 hours with vehicle, mepyramine or ranitidine prior to MTS assay and real-time PCR. Invasion was measured using a commercially available kit following vehicle, H1HR or H2HR antagonist treatment. The QCM ECMatrix Cell Invasion Assay was purchased from EMD Millipore (Billerica, MA) and used per manufacturer’s instructions (13 (link)).
