MC3T3-E1 Subclone 4 osteoblast cell lines were acquired from ATCC (ATCC CRL-2593). Cells were cultured in αMEM + Glutamax (32571-036; Gibco) supplemented with 10% FBS (Sigma-Aldrich) and 1% Pen/Strep (Corning). To stimulate procollagen synthesis and secretion, ascorbic acid 2-phospate (Sigma-Aldrich) was supplemented 18–24 h before imaging experiments.
Primary osteoblasts were extracted from mice harboring the G610C mutation and their wild-type littermates (B6.129(FVB)-Col1a2tm1Mcbr/J; Jackson Laboratories), which were maintained on the C57BL/6J background (4 (link), 23 (link)). Osteoblasts were extracted from parietal bones of 3- to 8-d-old mice as previously described (4 (link)). All care and procedures were performed in accordance with a Eunice Kennedy Shriver National Institute of Child Health and Human Development Animal Care and Use Committee-approved protocol.
Primary osteoblasts were extracted from mice harboring the G610C mutation and their wild-type littermates (B6.129(FVB)-Col1a2tm1Mcbr/J; Jackson Laboratories), which were maintained on the C57BL/6J background (4 (link), 23 (link)). Osteoblasts were extracted from parietal bones of 3- to 8-d-old mice as previously described (4 (link)). All care and procedures were performed in accordance with a Eunice Kennedy Shriver National Institute of Child Health and Human Development Animal Care and Use Committee-approved protocol.
