Investigating COX-2 Inhibition in Acute Cystitis

To test the effect of COX-2 inhibition on acute cystitis, mice were pretreated with SC-236 (Sigma) as previously described^{10 (link)}. The drug was solvated in 1% Tween 80 and mice received 100 µl drug, or buffer alone, per 20 g body weight by oral gavage. Mice were humanely euthanized at 24 h.p.i. with 10⁷ c.f.u. UTI89 and bladder bacterial burden was determined.

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O’Brien V.P., Hannan T.J., Yu L., Livny J., Roberson E.D., Schwartz D.J., Souza S., Mendelsohn C.L., Colonna M., Lewis A.L, & Hultgren S.J. (2016). A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease. Nature microbiology, 2, 16196.

Publication 2016

SC-236

Bacterial Bladder Body weight Buffer Cox 2 Cystitis Drug Gavage Inhibition Mice Sc 236 Tween 80

Corresponding Organization :

Other organizations : Washington University in St. Louis, Broad Institute, Massachusetts Institute of Technology, Columbia University

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Variable analysis

independent variables

Treatment with SC-236 (Sigma)

dependent variables

Bladder bacterial burden

control variables

Pretreatment with 1% Tween 80 buffer
Infection with 10^7 c.f.u. UTI89

controls

Positive control: Mice treated with SC-236 drug
Negative control: Mice treated with 1% Tween 80 buffer

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