Ethical Animal Experiments for Bordetella

This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committees at The University of Georgia at Athens, GA (A2022 04-001-Y1-A0 Bordetella-Host Interactions, A2022 04-025-Y1-A0 Breeding Protocol, and A2022 04-022-Y1-A0 Neonatal Models of Bordetella infection, transmission, and immunity). Mice were consistently monitored for signs of distress over the course of the experiments to be removed from the experiment and euthanized using carbon dioxide inhalation to prevent unnecessary suffering.