Deduced amino acid sequences of eomes genes were downloaded from the Ensembl genome browser (release 95, January 2019) for representative vertebrates, including human (Homo sapiens), mouse, chicken, and zebrafish. Sequence data of three other fishes were downloaded from species-specific genome databases, including grass carp (Ctenopharyngodon idella) (http://www.ncgr.ac.cn/grasscarp /), goldfish (https://research.nhgri.nih.gov/goldfish /), and common carp (http://www.fishbrowser.org/database/Commoncarp_genome /) (S2 Table ).
Protein sequences of eomes gene from aforementioned species were aligned by ClustalW2 with default parameters [35 (link)]. A phylogenetic tree was built, using a JTT substitution model with maximum likelihood (ML) method with 1,000 bootstrap replications in MEGA6 software package [36 (link)].
Protein sequences of eomes gene from aforementioned species were aligned by ClustalW2 with default parameters [35 (link)]. A phylogenetic tree was built, using a JTT substitution model with maximum likelihood (ML) method with 1,000 bootstrap replications in MEGA6 software package [36 (link)].
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