A duplicate set of six whole-milled kernels without cracks was selected and placed in a plastic box (5 cm × 5 cm × 2.5 cm). Approximately, 10 mL of 1.7% (0.3035 M) potassium hydroxide (KOH) solution was added. The samples were arranged to provide enough space between kernels to allow for spreading. The boxes were covered and incubated for 23 h in a 30°C oven. Starchy endosperm was rated visually based on a seven-point numerical spreading scale (43 ).
Gautam R.K., Singh P.K., Venkatesan K., Rakesh B., Sakthivel K., Swain S., Srikumar M., Zamir Ahmed S.K., Devakumar K., Rao S.S., Vijayan J., Ali S, & Langyan S. (2023). Harnessing intra-varietal variation for agro-morphological and nutritional traits in a popular rice landrace for sustainable food security in tropical islands. Frontiers in Nutrition, 10, 1088208.
Starchy endosperm spreading (rated on a seven-point numerical scale)
control variables
Duplicate set of six whole-milled kernels without cracks
Volume of 1.7% (0.3035 M) potassium hydroxide (KOH) solution (approximately 10 mL)
Arrangement of kernels to provide enough space for spreading
controls
No positive or negative controls were explicitly mentioned in the provided information.
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