Proteasome and Lysosome Activities in Fly Tissues

ROS levels, 26S CT-L and C-L proteasome activity levels, and cathepsins’ activity in total protein lysates isolated from somatic or muscle tissues of 10 flies were measured as described before [30 (link)]. Circulating or tissue sugar levels [trehalose (TRE), glucose (GLU), and glycogen (GLY)] were measured as reported before [31 (link)]. For all measurements, fluorescence or absorbance was recorded in a Spark^® Tecan microplate reader (Tecan Group Ltd., Maennedorf, Switzerland) and expressed as (%) values vs. the respective control groups set to 100%.

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Manola M.S., Gumeni S, & Trougakos I.P. (2021). Differential Dose- and Tissue-Dependent Effects of foxo on Aging, Metabolic and Proteostatic Pathways. Cells, 10(12), 3577.

Publication 2021

Spark® Tecan microplate reader

Cathepsins Flies Fluorescence Glucose Glycogen Muscle tissues Proteasome Protein Somatic Sugar Tissue Trehalose

Corresponding Organization : National and Kapodistrian University of Athens

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Variable analysis

independent variables

ROS levels
26S CT-L and C-L proteasome activity levels
Cathepsins' activity

dependent variables

Circulating or tissue sugar levels [trehalose (TRE), glucose (GLU), and glycogen (GLY)]

control variables

Somatic or muscle tissues of 10 flies

controls

Respective control groups set to 100%

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