ESBL Detection in Bacterial Isolates

Cefotaxime and ceftazidime were used as the initial screening standards, and strains resistant to both cefotaxime and ceftazidime were selected for ESBL screening. The double-disk synergy method was designed for drug susceptibility testing. ESBLs have the property of being inhibited by clavulanate. Bacteriostatic rings were found in resistant strains around the mixed discs of third-generation cephalosporins and clavulanate. If clavulanate increased the bacteriostatic ring by more than 5 mm, the isolates were presumed to produce ESBLs. Escherichia coli (ATCC 25922) and Klebsiella quasipneumoniae (ATCC 700603) were used as negative and positive controls, respectively.

Free full text: Click here

Shi W., Tang W., Li Y., Han Y., Cui L, & Sun S. (2023). Comparative Analysis between Salmonella enterica Isolated from Imported and Chinese Native Chicken Breeds. Microorganisms, 11(2), 390.

Publication 2023

Cefotaxime Ceftazidime Cephalosporins Clavulanate Escherichia coli Klebsiella quasipneumoniae Strains Susceptibility

Corresponding Organization : Shandong Agricultural University

Other organizations : Shandong Institute for Product Quality Inspection

Top 5 similar protocols

Variable analysis

independent variables

Cefotaxime
Ceftazidime

dependent variables

ESBL production by strains
Increase in bacteriostatic ring size around mixed discs of third-generation cephalosporins and clavulanate

control variables

Clavulanate

positive control

Klebsiella quasipneumoniae (ATCC 700603)

negative control

Escherichia coli (ATCC 25922)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!