SARS-CoV-2 HR2P Binding Kinetics

Real-time binding kinetics between 5-Helix and biotinylated SARS-CoV-2 HR2P was recorded on an Octet QK system (Fortebio, San Francisco, CA, USA) at 25 °C. Biotinylated SARS-CoV-2 HR2P was loaded onto a streptavidin biosensor. Serially diluted 5-Helix was associated with HR2P for 1200 s, followed by another 1200 s dissociation time in PBS containing 0.02% Tween. The equilibrium dissociation constant (K_D) was calculated by Octet QK software by a 1:1 binding model [16 (link)].

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Xing L., Xu X., Xu W., Liu Z., Shen X., Zhou J., Xu L., Pu J., Yang C., Huang Y., Lu L., Jiang S, & Liu S. (2022). A Five-Helix-Based SARS-CoV-2 Fusion Inhibitor Targeting Heptad Repeat 2 Domain against SARS-CoV-2 and Its Variants of Concern. Viruses, 14(3), 597.

Publication 2022

QK system

Biosensor Helix Kinetics Sars cov 2 Streptavidin Tween

Corresponding Organization : Southern Medical University

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Variable analysis

independent variables

Serially diluted 5-Helix

dependent variables

Real-time binding kinetics between 5-Helix and biotinylated SARS-CoV-2 HR2P
Equilibrium dissociation constant (KD)

control variables

Temperature (25 °C)
Buffer (PBS containing 0.02% Tween)
Biotinylated SARS-CoV-2 HR2P loaded onto a streptavidin biosensor

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