Xenograft Tumor Growth Assay in Mice

T47D:A18/PKCα, T47D:A18/neo andT47D:A18-TAM1 tumors were established as previously described (7 (link)). E2 was administered via silastic capsules (1.0 cm) implanted subcutaneously between the scapulae, producing a mean serum E2 level of 379.5 pg/mL (11 (link), 15 (link)). BTC and TTC-352 were administered p.o. at a dose of 1.5 mg/animal daily for 2 weeks as previously described for other SERMs (7 (link)). RAL was administered p.o. at a dose 1.5 mg/animal daily for 2 weeks. Tumor cross-sectional area was determined weekly using Vernier calipers and calculated using the formula: length/2 × width/2 × π. Mean tumor area was plotted against time in weeks to monitor tumor growth. The mice were sacrificed by CO₂ inhalation and cervical dislocation, and tumors and uteri were excised, cleaned of connective tissue, and immediately weighed. The Animal Care and Use Committee of the University of Illinois at Chicago approved all of the procedures involving animals.

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Molloy M.E., Perez White B.E., Gherezghiher T., Michalsen B.T., Xiong R., Patel H., Zhao H., Maximov P.Y., Jordan V.C., Thatcher G.R, & Tonetti D.A. (2014). Novel Selective Estrogen Mimics for the Treatment of Tamoxifen-Resistant Breast Cancer. Molecular cancer therapeutics, 13(11), 2515-2526.

Publication 2014

Animals Capsules Cervical Connective tissue Dislocation Inhalation Mice Pkcα Scapulae Serms Serum Silastic Tumors Uteri

Corresponding Organization :

Other organizations : University of Illinois at Chicago, Georgetown University Medical Center, Georgetown University

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Protocol cited in 5 other protocols

Variable analysis

independent variables

BTC administration (1.5 mg/animal daily for 2 weeks)
TTC-352 administration (1.5 mg/animal daily for 2 weeks)
RAL administration (1.5 mg/animal daily for 2 weeks)

dependent variables

Tumor cross-sectional area (measured weekly)
Tumor weight (measured at the end of the experiment)
Uterus weight (measured at the end of the experiment)

control variables

T47D:A18/PKCα tumors
T47D:A18/neo tumors
T47D:A18-TAM1 tumors
Estradiol (E2) administration via subcutaneous silastic capsules (1.0 cm), producing a mean serum E2 level of 379.5 pg/mL

positive controls

T47D:A18/PKCα tumors

negative controls

T47D:A18/neo tumors
T47D:A18-TAM1 tumors

Annotations

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