Publication quality images of injection sites and labeled fibers were captured using a Spotflex 64 mega pixel camera, (Diagnostic Instruments Inc., Sterling Heights, MI, USA, version 4.6), mounted on an Olympus BX51 microscope. Photographic montages of the injection sites and labeled fibers were created using Adobe PhotoShop 7.0 (Adobe Systems Inc., San Jose, CA, USA). Only brightness and contrast were adjusted to maximize discrimination and normalize images for comparative purposes. Cortical reconstructions and reconstruction of the physiological stimulation maps were developed as previously described using metrically calibrated digital images of the cortical surface (Morecraft and Van Hoesen 1992 (link), Morecraft et al., 2002 (link), 2013 (link)). Publication quality line illustrations were created using Adobe Illustrator 10.0 (Adobe Systems Inc., San Jose, CA, USA).
Protocol detail
Cortical Mapping Using Photographic Techniques
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Cortical
Diagnostic
Discrimination
Injection sites
Maps
Microscope
Physiological
Reconstruction
Corresponding Organization : University of South Dakota
Other organizations : University of Iowa
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Variable analysis
independent variables
- None explicitly mentioned
- Publication quality images of injection sites
- Publication quality images of labeled fibers
- Brightness and contrast were adjusted to maximize discrimination and normalize images for comparative purposes
- Positive controls: None mentioned
- Negative controls: None mentioned
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