Tau ELISAs were performed on Hank’s balanced salt solution (HBSS)
without with Ca2+ and Mg2+ medium that had been incubated
for 4 hours with 22-23 DIV primary neurons as we described previously (Croft et al., 2017 (link)).
Lactate dehydrogenase amounts in the media of cultured neurons was determined as
a measure of neuron heath, using an LDH cytotoxicity kit from Thermo Fischer
Scientific according to the manufacturer’s instructions.
without with Ca2+ and Mg2+ medium that had been incubated
for 4 hours with 22-23 DIV primary neurons as we described previously (Croft et al., 2017 (link)).
Lactate dehydrogenase amounts in the media of cultured neurons was determined as
a measure of neuron heath, using an LDH cytotoxicity kit from Thermo Fischer
Scientific according to the manufacturer’s instructions.
