Fructosamine (Roche Diagnostics Corp, Indianapolis, IN, USA) and glycated albumin (Lucia GA-L, Asahi Kasei Corp, Tokyo, Japan) were measured in 2012–2013 from serum samples, using a Roche Modular P800 system. Samples had been stored at −70°C since collection during the baseline examination in 1990 to 1992. For Fructosamine, the inter-assay coefficients of variation (CVs) were 3.2% at a concentration of 212.6 umol/L and 2.5% at a concentration of 856.7 umol/L%. For glycated albumin, the inter-assay CVs were 2.3% at a concentration of 1.579 g/dL and 2.8% at a concentration of 0.426 g/dL. Previous studies have demonstrated the stability of these analytes in long-term stored samples7 (link)–9 (link).