Culturing High-Grade Serous Carcinoma Cell Lines

All reagents were obtained from Life Technologies (Carlsbad, CA) unless otherwise indicated. Murine oviductal cells (MOE) and murine ovarian surface epithelial cells (MOSE) were obtained from Dr. Barbara Vanderhyden at the University of Ottawa. MOE and MOSE cells were cultured as previously described [34 (link)]. OVCAR4 cells were obtained from the National Cancer Institute from the Division of Cancer Treatment and Diagnosis Tumor Repository. Kuramochi cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB). OVCAR4 and Kuramochi cells were cultured using RPMI 1640 media, supplemented with 10% FBS (Denville Scientific, Holliston, MA) and 1% pen/strep. OVCAR3 cells were purchased from the American Type Culture Collection (ATCC). OVCAR3 cells were cultured using MEM, supplemented with 20% FBS (Denville Scientific, Holliston, MA), 0.05 mg/mL Insulin, 1% non-essential amino acids, 1% sodium pyruvate, 1% L-glutamine, and 1% pen/strep. OVCAR3 and Kuramochi cells have been verified by STR analysis. The molecular profiles and in-vivo tumor growth capabilities of all three HGSC lines used in this study have been previously characterized [35 (link)–37 ].