Characterization of Cell Lines

HEK293 (ATCC), MCF-10A (ATCC), primary Schwann (ScienCell), and immortalized human normal Schwann cells (Margaret Wallace, University of Florida)^{63 (link)} were authenticated using short tandem repeat profiling at MSKCC IGO and tested for mycoplasma using the PCR-based Universal Mycoplasma Detection kit (ATCC). HEK293 and immortalized Schwann cells were cultured in Dulbecco's modified Eagle's medium (DMEM) high glucose supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. MCF-10A cells were cultured in DMEM/F12 supplemented with 5% horse serum, 20 ng/ml epidermal growth factor, 10 μg/ml insulin, 0.5 μg/ml hydrocortisone, and 1% penicillin/streptomycin. Primary Schwann cells were cultured in Schwann Cell Medium (ScienCell), consisting of basal medium, 5% FBS, 1% Schwann cell growth supplement, and 1% penicillin/streptomycin. All cell lines were maintained in a 5% CO₂ atmosphere at 37 °C.