Measuring Serum hCG Levels Using Immulite 2000 XPi

hCG was analyzed in serum using a solid-phase two-site chemiluminescent immunometric assay, calibrated against WHO 3rd IS 75/537, on an Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA). The Siemens assay detects serum intact hCG, hyperglycosylated hCG, serum nicked hCG, serum nicked hyperglycosylated hCG, serum asialo hCG, serum hCG free β-subunit and serum nicked hCG β [25 (link)]. The inter assay coefficient of variation was 8.0, 6.3 and 5.1 % at the concentration of 9.7, 53.1 and 821.5 IU/L, respectively. Although the Immulite 2000 is considered as one of the best assays for total hCG, it should be noted that the reference ranges in this paper are assay specific and do not correspond with hCG values obtained from different assays [26 ].

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Korevaar T.I., Steegers E.A., de Rijke Y.B., Schalekamp-Timmermans S., Visser W.E., Hofman A., Jaddoe V.W., Tiemeier H., Visser T.J., Medici M, & Peeters R.P. (2015). Reference ranges and determinants of total hCG levels during pregnancy: the Generation R Study. European Journal of Epidemiology, 30(9), 1057-1066.

Publication 2015

Immulite 2000 XPi system

Asialo hcg Assays Chemiluminescent assay Diagnostics Hcg β subunit Serum

Corresponding Organization :

Other organizations : Erasmus University Rotterdam, Erasmus MC - Sophia Children’s Hospital, Erasmus MC

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum hCG levels

control variables

WHO 3rd IS 75/537 used for calibration of the Immulite 2000 XPi system
The Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA) was used to analyze serum hCG
The inter-assay coefficient of variation was reported at 8.0%, 6.3%, and 5.1% at hCG concentrations of 9.7, 53.1, and 821.5 IU/L, respectively

controls

No positive or negative controls were explicitly mentioned

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