Imaging was performed on a 9.4-Tesla small animal imaging system controlled by an Agilent VnmrJ 4 console. A custom-made silver solenoid coil was used for radiofrequency transmission and reception. Diffusion imaging was accomplished using a 3D diffusion-weighted spin-echo pulse sequence with repetition time (TR) = 100 ms, echo time (TE) = 15 ms, and b-value = 4000 s/mm2. The acquisition matrix was 568 × 284 × 228 over a 24.4 mm × 12.2 mm × 9.8 mm field of view, resulting in a native isotropic image resolution of 43 µm. The diffusion sampling protocol included 120 unique diffusion directions (Koay 2011 (link); Koay et al. 2011 (link)) and 11 nondiffusion-weighted (b0) measurements (i.e., one b0 every 12 diffusion measurements). Total acquisition time was 235 h. Bore temperature was controlled with a chilled water pump and monitored with a fiber-optic probe for the duration of the experiment (Supplementary Fig. 1).