Blood Lipid Profiling Protocol

Venous blood samples of 5 ml were drawn after at least 12 h of fasting. A total of 2 ml of the sample was collected into a glass tube and used to determine serum lipid levels. The remaining 3 ml was transferred to tubes with anticoagulants (4.80 g/l citric acid, 14.70 g/l glucose and 13.20 g/l trisodium citrate; Shanghai Sangon Biological Engineering Technology & Services Co.) and used to extract DNA. Measurements of serum TC, TG, HDL-C and LDL-C levels in the samples were performed using enzymatic methods with a Ransod autoanalyzer (Randox Laboratories Ltd., Crumlin, UK and Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan). Serum ApoA1 and ApoB levels were detected using the immunoturbidimetric immunoassay using a commercial kit (Randox Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University (27 (link)–30 (link)).

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GUO T., YIN R.X., WU J., LIN Q.Z., SHI G.Y., SHEN S.W., SUN J.Q., LI H., LIN W.X, & YANG D.Z. (2015). Association of the angiopoietin-like protein 8 rs2278426 polymorphism and several environmental factors with serum lipid levels. Molecular Medicine Reports, 12(3), 3285-3296.

Publication 2015

citric acid trisodium citrate Type 7170A

Anticoagulants Apoa1 Apob Blood Citric acid Enzymatic Glucose Immunoassay Immunoturbidimetric Lipid Randox Serum Trisodium citrate Venous

Corresponding Organization :

Other organizations : First Affiliated Hospital of GuangXi Medical University, Guangxi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum TC (total cholesterol) levels
Serum TG (triglyceride) levels
Serum HDL-C (high-density lipoprotein cholesterol) levels
Serum LDL-C (low-density lipoprotein cholesterol) levels
Serum ApoA1 (apolipoprotein A1) levels
Serum ApoB (apolipoprotein B) levels

control variables

Venous blood samples of 5 ml were drawn after at least 12 h of fasting
2 ml of the sample was collected into a glass tube
3 ml of the sample was transferred to tubes with anticoagulants (4.80 g/l citric acid, 14.70 g/l glucose and 13.20 g/l trisodium citrate)

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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