Histology was performed as previously described (Gaffin et al., 2011 (link)) with some slight modifications. Mice were anesthetized using 200 mg ketamine/20 mg xylazine per kg body weight. The hearts were quickly removed and retrogradely perfused through the aorta with ice-cold saline followed by 10% formalin. The heart was then transversely sliced into four pieces, and each piece was placed into a cassette. Cassettes were kept in formalin and shipped to the Veterinary Diagnostic Laboratory at the University of Illinois at Urbana-Champaign (UIUC) College of Veterinary Medicine for paraffin embedding and sectioning. Sections (5 μm) were placed on slides and stained using hematoxylin and eosin (H&E) and Masson’s trichrome by conventional methods. Sections were imaged with a microscope coupled to a camera and viewed using Leica Aperio ImageScope software.
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