Biomarker Profiling in Fasting Blood

Venous blood samples were collected after 8 h of overnight fasting. Fasting plasma concentrations of glucose, TGs, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL) cholesterol were determined by a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). Glycated hemoglobin (HbA1c) levels were determined by high-performance liquid chromatography with an automated HLC-723G7 analyzer (Tosoh Corporation, Tokyo, Japan). Serum creatinine levels were measured colorimetrically (Hitachi Automatic Analyzer 7600), and eGFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration equation [21 (link)]. To obtain the UACR, urinary albumin was measured in fasting morning spot urine using the immunoturbidimetric method, and urinary creatinine was measured using the colorimetric method. Urine sodium concentrations were measured using an ion-selective electrode, and 24-h sodium excretion was estimated based on concentrations of sodium and creatinine in fasting morning spot urine specimens according to 3 different methods (S1 Table).

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Choi J.W., Park J.S, & Lee C.H. (2020). Interactive effect of high sodium intake with increased serum triglycerides on hypertension. PLoS ONE, 15(4), e0231707.

Publication 2020

Hitachi Automatic Analyzer 7600 HLC-723G7 analyzer

Albumin Cholesterol Chronic kidney disease Colorimetric Creatinine Egfr Glucose Glycated hemoglobin High density lipoprotein cholesterol High performance liquid chromatography Immunoturbidimetric Ion selective electrode Low density lipoprotein Plasma Serum Sodium Urine Venous blood

Corresponding Organization : Hanyang University

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Variable analysis

independent variables

Overnight fasting for 8 hours

dependent variables

Fasting plasma concentrations of glucose, TGs, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL) cholesterol
Glycated hemoglobin (HbA1c) levels
Serum creatinine levels
Estimated glomerular filtration rate (eGFR)
Urine albumin-to-creatinine ratio (UACR)
Urine sodium concentrations
24-h sodium excretion

control variables

Venous blood samples were collected after 8 h of overnight fasting
Measurements were conducted using standardized analytical methods and equipment (Hitachi Automatic Analyzer 7600, Tosoh Corporation HLC-723G7 analyzer)

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