Culturing Campylobacter jejuni under Various Conditions

Bacterial strains used in this study are listed in Table 1. Campylobacter jejuni strains were cultured on Mueller-Hinton (MH) agar under microaerobic conditions (85% N₂, 10% CO₂, 5% O₂) at 42°C. Incubation at 37°C was performed for assays that included comparison between temperatures. Oxygen-limited/anaerobic conditions were achieved using the BD GasPak Sachets system (BD diagnostics, Franklin Lakes, NJ) as described previously (Kassem et al. 2012 (link)). Different oxygen conditions and/or temperatures were used in some assays to be inclusive of varying conditions encountered by C. jejuni in disparate hosts and niches (Kassem et al. 2012 (link)). Laked horse blood (5%, Oxoid, Lenexa, KS), antibiotics (chloramphenicol: 20 μg mL⁻¹, kanamycin: 50 μg mL⁻¹), and the Campylobacter selective supplement (SR155E, Oxoid) were added to the MH medium when necessary.