Immunostaining of Human Tissue Sections

All stains were performed on 7 μm cryomicrotome sections, according to standard procedures. Immunohistochemical and double immunofluorescence reactions were carried out as described previously (13 (link)). The following antibodies were used for staining procedures:
Mouse anti-human CD163, 1:50, St. John's Lab/polyclonal; rabbit anti-human CD74, 1:100 St. John's Lab/polyclonal; mouse anti-human CD68, 1:100 Dako/EBM1; rabbit anti-human iNOS, ready-to-use, Genetex/polyclonal; rabbit anti-human ISG15, 1:100, abcam/polyclonal; MHCI, 1:1.000, Dako/W6/32; mouse anti-human MHC class II, 1:100, DAKO/ CR3/43; rat anti-human STAT1, 1:50, R&D Systems/246523; mouse anti-human STAT6, 1:50, R&D Systems/253906; mouse anti-human Siglec1, 1:100, Novus Biologicals/HSn7D2; IRF8, 1:100 Abcam/polyclonal.

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Roos A., Preusse C., Hathazi D., Goebel H.H, & Stenzel W. (2019). Proteomic Profiling Unravels a Key Role of Specific Macrophage Subtypes in Sporadic Inclusion Body Myositis. Frontiers in Immunology, 10, 1040.

Publication 2019

mouse anti-human CD68

Antibodies Biologicals Cd163 Human Immunofluorescence Inos Irf8 Mhc class ii Mouse Novus Rabbit Stat1 Stat6

Corresponding Organization :

Other organizations : Leibniz Institute for Analytical Sciences - ISAS, University of Duisburg-Essen, Humboldt-Universität zu Berlin, Freie Universität Berlin, Charité - Universitätsmedizin Berlin

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All stains were performed on 7 μm cryomicrotome sections, according to standard procedures.
Immunohistochemical and double immunofluorescence reactions were carried out as described previously (13 (link)).

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