Murine Models for Autoimmune Diseases

C57BL/6 congenic mice (B6 Thy1.2; Thy1.1⁺) and NSG (NOD-scid IL2Rg^null) mice were attained from The Jackson Laboratory (Bar Harbor, Maine, USA). eEF2K^-/- mice (C57BL/6 background; Thy1.2⁺)^{47 (link)} were generous gifts from Dr. Alexey Ryazonov (Rutgers University, Robert Wood Johnson Medical School, New Jersey). All mice were maintained under pathogen-free conditions, with a 12:12-hour light-dark cycle, within the animal facility of the Texas A&M University. NSG (NOD-scid IL2Rg^null). C57BL/6 congenic mice and eEF2K deficient mice (6–8 weeks, male) were used for the murine CIA model. NSG (NOD-scid IL2Rg^null) mice were used for the T cell transfer colitis model. C57BL/6 congenic mice and eEF2K deficient mice (9–13 weeks, female) were used for the multiple sclerosis model. All mice used for the rest of the experiments were male and female, aged 5–10 weeks.