Protocol detail

Viral RNA Extraction and Quantification

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Viral RNAs were extracted using QIAamp viral RNA (Qiagen) and reverse transcriptions were performed using a PrimeScript RT reagent kit with a gDNA Eraser (Takara Bio, Inc., Kusatsu, Shiga Prefecture, Japan). Viral RNA in animal samples were quantified by QuantiNova Probe RT-PCR kit (QIAGEN, Inc., Hilden, Germany) using primers targeting RNA-dependent RNA polymerase (RdRp). Taqman probe and qPCR primers were adopted from previous studies [10 (link)].

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Lam J.Y., Wong W.M., Yuen C.K., Ng Y.Y., San C.H., Yuen K.Y, & Kok K.H. (2023). An RNA-Scaffold Protein Subunit Vaccine for Nasal Immunization. Vaccines, 11(10), 1550.

Publication 2023

QIAamp viral RNA PrimeScript RT reagent kit gDNA Eraser QuantiNova Probe RT-PCR kit

Animal Primers Reverse transcriptions Rna dependent rna polymerase Rt pcr Viral rna

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Variable analysis

independent variables

Viral RNA extraction method (QIAamp viral RNA)

dependent variables

Viral RNA quantification in animal samples

control variables

Reverse transcription method (PrimeScript RT reagent kit with a gDNA Eraser)
Quantification method (QuantiNova Probe RT-PCR kit)
Primers targeting RNA-dependent RNA polymerase (RdRp)
Taqman probe and qPCR primers adopted from previous studies [10]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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