Maintaining B. malayi Life Cycle in Gerbils

The B. malayi (Bm) filarial nematode parasite life cycle [TRS strain (63 (link))] was maintained in mosquitoes and susceptible M. unguiculatus gerbils at Liverpool School of Tropical Medicine (LSTM) or TRS laboratories. Infective Bm larvae (BmL3) were bred via procedures as previously described (64 (link)). Briefly, mf collected from infected gerbils by catheterization (65 (link)) were fed to female adult Aedes aegypti; mf were mixed with human blood and fed to mosquitoes using an artificial membrane feeder (Hemotek). BmL3-stage larvae were propagated by rearing the blood-fed mosquitoes for 14 d. BmL3 larvae were then harvested from infected mosquitoes by crushing and purification (64 (link)).

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Turner J.D., Sharma R., Al Jayoussi G., Tyrer H.E., Gamble J., Hayward L., Priestley R.S., Murphy E.A., Davies J., Waterhouse D., Cook D.A., Clare R.H., Cassidy A., Steven A., Johnston K.L., McCall J., Ford L., Hemingway J., Ward S.A, & Taylor M.J. (2017). Albendazole and antibiotics synergize to deliver short-course anti-Wolbachia curative treatments in preclinical models of filariasis. Proceedings of the National Academy of Sciences of the United States of America, 114(45), E9712-E9721.

Publication 2017

artificial membrane feeder

Adult female Aedes Artificial membrane Blood Catheterization Gerbils Human Larvae Mosquitoes Nematode Parasite Strain

Corresponding Organization :

Other organizations : Liverpool School of Tropical Medicine

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Variable analysis

independent variables

Procedures for breeding infective Bm larvae (BmL3)

dependent variables

Propagation of BmL3-stage larvae

control variables

Maintenance of B. malayi (Bm) filarial nematode parasite life cycle in mosquitoes and susceptible M. unguiculatus gerbils
Collection of microfilariae (mf) from infected gerbils by catheterization
Feeding of mf to female adult Aedes aegypti mosquitoes
Rearing of blood-fed mosquitoes for 14 days
Harvesting of BmL3 larvae from infected mosquitoes by crushing and purification

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