Differentiation of FOXA2 Knockout iPSCs into Pancreatic Progenitors

iPSC lines (Ctr1-iPSCs and Ctr2-iPSCs) generated and fully characterized in our laboratory were used as we previously reported [32 (link)]. FOXA2 knockout iPSCs from Ctr1-and Ctr2-iPSCs were generated using CRISPR/Cas9 as we recently reported [5 (link)]. Both wild-type (WT) and FOXA2^–/– iPSCs were cultured and maintained using Stemflex media (ThermoFisher Scientific) on Matrigel-coated plates (Corning). iPSC lines were differentiated into PPs using our established protocol (Supplementary Table 1) [33 (link)–35 (link)].

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Aldous N., Elsayed A.K., Alajez N.M, & Abdelalim E.M. (2023). iPSC-Derived Pancreatic Progenitors Lacking FOXA2 Reveal Alterations in miRNA Expression Targeting Key Pancreatic Genes. Stem Cell Reviews and Reports, 19(4), 1082-1097.

Publication 2023

Stemflex media Matrigel-coated plates

Crispr Ipsc Matrigel

Corresponding Organization :

Other organizations : Hamad bin Khalifa University, Qatar Foundation

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Variable analysis

independent variables

Knockout of FOXA2 gene in iPSCs using CRISPR/Cas9

dependent variables

Differentiation of iPSCs into pancreatic progenitors (PPs)

control variables

Culture and maintenance of wild-type (WT) and FOXA2-/- iPSCs using Stemflex media on Matrigel-coated plates
Established protocol for differentiating iPSCs into PPs

controls

Wild-type (WT) iPSCs as a positive control

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