Immunohistochemical Analysis of RANK and RANKL in Gastric Carcinoma

Formalin-fixed, paraffin-embedded primary gastric carcinoma tissues were cut into 3-mm sections. The IHC method is discussed in our previous study (20 (link)). S-P immunohistochemical kit and 30-diamino-benzidine tetrahydrochloride (DAB) kit were obtained from Maixin Bio (Fuzhou Maixin Biological Technology Ltd., China). Immunohistochemical staining was performed using the following antibodies: anti-RANK antibody from RD Company and anti-RANKL antibody from Abcam (USA). Sections were observed through microscopy (×20 and ×40) by two independent pathologists. From each section, 5 visual fields were randomly selected and scoring was done according to the percentage of positive cells and the staining intensity. Positive cells of <10, 10-25, 26-50, 51-75, >76% were recorded as 0, 1, 2, 3, and 4, respectively. A score >2 was considered as high expression, 0-2 as low expression.

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Zhang X., Song Y., Song N., Zhang L., Wang Y., Li D., Wang Z., Qu X, & Liu Y. (2018). Rankl expression predicts poor prognosis in gastric cancer patients: results from a retrospective and single-center analysis. Brazilian Journal of Medical and Biological Research, 51(3), e6265.

Publication 2018

S-P immunohistochemical kit anti-RANKL antibody

Anti antibody Antibodies Benzidine Biological Carcinoma Cells Formalin Gastric Microscopy Paraffin Pathologists Rankl Tissues

Corresponding Organization :

Other organizations : First Hospital of China Medical University, China Medical University

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Variable analysis

independent variables

Antibodies: anti-RANK antibody from RD Company and anti-RANKL antibody from Abcam (USA)

dependent variables

Expression of RANK and RANKL proteins in primary gastric carcinoma tissues

control variables

Formalin-fixed, paraffin-embedded primary gastric carcinoma tissues
3-mm tissue sections
S-P immunohistochemical kit and 30-diamino-benzidine tetrahydrochloride (DAB) kit from Maixin Bio (Fuzhou Maixin Biological Technology Ltd., China)
Scoring based on the percentage of positive cells and the staining intensity
Microscopic observation (×20 and ×40) by two independent pathologists

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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