Grouper Spleen Cells SGIV Infection

Grouper spleen cells were cultured to 100% confluence in 12-well plates or 35-mm glass-bottom dishes (Cellvis, Hangzhou, China; catalog number D35-14-1-N) at 28°C for 24 h. After the cells were infected with SGIV-Gx at a multiplicity of infection (MOI) of 1 at 28°C for 6 h, they were then washed with phosphate-buffered saline (PBS: 10 mM Na₂HPO₄⋅12H₂O, 2 mM KH₂PO₄, 137 mM NaCl, 1% NaN₃) and collected for further use (Yu et al., 2019a (link)).

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Yu Q., Liu M., Wu S., Wei X., Xiao H., Yi Y., Cheng H., Wang S., Zhang Q., Qin Q, & Li P. (2020). Specific Aptamer-Based Probe for Analyzing Biomarker MCP Entry Into Singapore Grouper Iridovirus-Infected Host Cells via Clathrin-Mediated Endocytosis. Frontiers in Microbiology, 11, 1206.

Publication 2020

35-mm glass-bottom dishes

Cells Dishes Infection Nacl Nan3 Phosphate Saline Spleen

Corresponding Organization : South China Agricultural University

Other organizations : Guangxi Academy of Fishery Sciences, Guangxi University of Science and Technology

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Variable analysis

independent variables

Multiplicity of infection (MOI) of SGIV-Gx virus

dependent variables

Cell response after SGIV-Gx virus infection

control variables

Grouper spleen cells
Culture conditions (12-well plates or 35-mm glass-bottom dishes, 28°C, 24 h)
Phosphate-buffered saline (PBS) composition (10 mM Na2HPO4·12H2O, 2 mM KH2PO4, 137 mM NaCl, 1% NaN3)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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