Nanoparticle Characterization: Size and Charge

Particle size distribution of the synthesized NPs was analyzed using dynamic light scattering on DynaPro-TC-04 DLS instrument (Protein Solutions, Wyatt Technology, Santa Barbara, CA) the size distribution of as-synthesized NPs was also observed by Nanophox particle size analyzer (Sympatec GmbH, Clausthal-Zellerfeld, Germany). To evaluate the stability and surface charge of the as synthesized 5-FU NPs, zeta potential was determined using DTS software (Malvern Instruments, Worcestershire, UK) based on M3-PALS technology following a method standardized in our laboratory^{38 (link)}. 5-FU NPs were suspended in phosphate buffer (10 mM, pH 7.4) and thoroughly mixed in a cyclo-mixer for 1 min and used for zeta sizing as well as surface charge determination.

Free full text: Click here

Mohammad O., Faisal S.M., Ahmad N., Rauf M.A., Umar M.S., Mujeeb A.A., Pachauri P., Ahmed A., Kashif M., Ajmal M, & Zubair S. (2019). Bio-mediated synthesis of 5-FU based nanoparticles employing orange fruit juice: a novel drug delivery system to treat skin fibrosarcoma in model animals. Scientific Reports, 9, 12288.

Publication 2019

Nanophox particle size analyzer DTS software

Buffer Pals Phosphate Protein

Corresponding Organization : Aligarh Muslim University

Other organizations : Jeddah University, National Institute of Immunology, Jawaharlal Nehru Medical College Hospital

Top 5 similar protocols

Variable analysis

independent variables

Particle size distribution analysis method (Dynamic light scattering on DynaPro-TC-04 DLS instrument and Nanophox particle size analyzer)

dependent variables

Particle size distribution of the synthesized NPs
Zeta potential of the as-synthesized 5-FU NPs

control variables

Phosphate buffer (10 mM, pH 7.4) used to suspend the 5-FU NPs
Cyclo-mixer used to thoroughly mix the 5-FU NPs in the phosphate buffer

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!