Inflammatory Signaling Pathway Analysis

Protein expression was analyzed by western blotting as previously described (Chai et al. 2014 (link); Yuan et al. 2015 (link)). For western blot analysis, primary antibodies against NFκB (rabbit polyclonal antibody, ab16502), TNFα (rabbit polyclonal antibody, ab6671), NLRP3 (rabbit polyclonal antibody, ab210491), IL-1β (rabbit polyclonal antibody, ab9722), IL-6 (rabbit polyclonal antibody, ab208113) and caspase1 (rabbit polyclonal antibody, ab1872) were purchased from Abcam (UK). TLR4 (mouse monoclonal antibody, sc-293072) and ASC (mouse monoclonal antibody, sc-271054) were purchased from Santa Cruz (USA). IL-18 (rabbit polyclonal antibody, TA324190) was purchased from ORIGENE (USA). The goat anti-mouse IgG-HRP (ZB2305) and goat anti-rabbit (ZB2301) IgG-HRP secondary antibodies were purchased from ZSGB-Bio, Beijing, China. The targeted proteins were visualized with the Super Signal West Femto Chemiluminescent Substrate (Thermo Scientific Pierce) and the intensity of the visualized bands were analyzed using Quantity One software (Bio-Rad). β-actin (mouse monoclonal antibody, TA-09, Zhongshan Jinqiao Biotech company, Beijing, China) was used as an internal control. Data are expressed as a ratio to β-actin.

Free full text: Click here

Gong Q., He L., Wang M., Zuo S., Gao H., Feng Y., Du L., Luo Y, & Li J. (2019). Comparison of the TLR4/NFκB and NLRP3 signalling pathways in major organs of the mouse after intravenous injection of lipopolysaccharide. Pharmaceutical Biology, 57(1), 555-563.

Publication 2019

ab16502 ab6671 ab210491 ab9722 ab208113 ab1872 sc-293072 sc-271054 TA324190 goat anti-mouse IgG-HRP (ZB2305) goat anti-rabbit (ZB2301) IgG-HRP secondary antibodies Super Signal West Femto Chemiluminescent Substrate Quantity One software

Actin Anti igg Antibodies Antibody Goat Igg hrp Il 18 Il 1β Monoclonal antibody Mouse Nfκb Proteins Rabbit Tnfα Western blot analysis

Corresponding Organization : Jiangxi University of Traditional Chinese Medicine

Other organizations : Guangxi University, Guangxi University of Chinese Medicine, Tsinghua University

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression of NFκB
Protein expression of TNFα
Protein expression of NLRP3
Protein expression of IL-1β
Protein expression of IL-6
Protein expression of caspase1
Protein expression of TLR4
Protein expression of ASC
Protein expression of IL-18

control variables

β-actin (used as an internal control)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!